Product Description
| Cat Number | IL1-MM112 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Mouse IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus of IL-12A.It contains Arg23-Ala215(IL-12A) and Met23-Ser335(IL-12B). |
| Target | IL-12 |
| Host | Mouse |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably. |
| Molecular Mass | The protein has a predicted MW of 22.80 kDa(IL-12A)&35.79 kDa(IL-12B). Due to glycosylation, the protein migrates to 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) based on Bis-Tris PAGE result. |
| Sl Sequence | Arg23-Ala215(IL-12A) & Met23-Ser335(IL-12B) |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | P43431(IL-12A)&P43432(IL-12B) |
| Amino Acid Sequence | Recombinant Mouse IL-12 Protein is expressed from HEK293 with His tag at the C-Terminus of IL-12A.It contains Arg23-Ala215(IL-12A) and Met23-Ser335(IL-12B). |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
Share Item: