Biotinylated Human IL-17A&F Protein (ILF-HM418B)
SKU: BTL-KB-P-02128 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | ILF-HM418B |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Biotinylated Human IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A cotains no tag and subunit IL-17F is fused with a His tag and a Avi tag at the C-terminus.It contains Gly24-Ala155 (IL-17A) and Arg31-Gln163 (IL-17F). |
| Target | IL-17A&F |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines. |
| Molecular Mass | The protein has a predicted MW of 15.1 kDa (IL-17A) and 17.5 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa and 27 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F) |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | Q16552-1(IL-17A)&Q96PD4(IL-17F) |
| Amino Acid Sequence | Recombinant Biotinylated Human IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A cotains no tag and subunit IL-17F is fused with a His tag and a Avi tag at the C-terminus.It contains Gly24-Ala155 (IL-17A) and Arg31-Gln163 (IL-17F). |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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