Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein (ITG-HM1MB)
SKU: BTL-KB-P-02212 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | ITG-HM1MB |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of ITGAM; It contains Phe17-Asn1104 (ITGAM) acidic tail and Gln23-Asn700 (ITGB2) basic tail. |
| Target | Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE |
| Scientific Background | Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DCSIGN[2]. |
| Molecular Mass | The protein has a predicted MW of 126.10 kDa (ITGAM) and 79.50 kDa (ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM) and 90-100 kDa (ITGB2) based on Bis-Tris PAGE result. |
| Sl Sequence | Phe17-Asn1104 (ITGAM) acidic tail & Gln23-Asn700 (ITGB2) basic tail |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | P11215-1(ITGAM)&P05107-1(ITGB2) |
| Amino Acid Sequence | Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus of ITGAM; It contains Phe17-Asn1104 (ITGAM) acidic tail and Gln23-Asn700 (ITGB2) basic tail. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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