Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein (MHC-HE013B)
SKU: BTL-KB-P-02572 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | MHC-HE013B |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide. |
| Target | HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population. |
| Molecular Mass | The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result. |
| Sl Sequence | Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | E.coli |
| Gene Id | A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV |
| Amino Acid Sequence | Recombinant Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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