Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein (MHC-HM406)
SKU: BTL-KB-P-02658 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | MHC-HM406 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-Terminus.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide. |
| Target | HLA-E*01:03&B2M&Peptide (VMAPRTLVL) |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes. |
| Molecular Mass | The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL |
| Amino Acid Sequence | Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Monomer Protein is expressed from HEK293 with His tag and Avi at the C-Terminus.It contains Gly22-Thr302(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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