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Human HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET) Monomer Protein (MHC-HM424)

SKU: BTL-KB-P-02720 | Host: Human | Brand: Kactus Bio
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Product Description

Cat Number MHC-HM424
Category Recombinant Protein
Pack Size 100UG
Description Recombinant Human HPV16 E7(HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YMLDLQPET peptide.
Target HLA-A*02:01&B2M&HPV16 E7 (YMLDLQPET)
Host Human
Purity > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Scientific Background HPV16 E7 protein, one of the primary target proteins in molecular targeted therapy for HPV-induced cervical cancer. The affitoxin, ZHPV16E7 affitoxin384 was generated by fusing the modified Pseudomonas Exotoxin A (PE38KDEL) to the HPV16 E7-specific affibody.
Molecular Mass The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Bis-Tris PAGE result.
Sl Sequence Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YMLDLQPET peptide
Solubility Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Appearance Lyophilized
Endotoxin Levels Less than 1 EU per μg by the LAL method.
Expression System HEK293
Gene Id P04439-1(HLA-A*02:01)&P61769(B2M)&YMLDLQPET peptide
Amino Acid Sequence Recombinant Human HPV16 E7(HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YMLDLQPET peptide.
Storage -20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Note The product is for research use only
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