Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer Protein (MHC-HM432T)
SKU: BTL-KB-P-02743 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | MHC-HM432T |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide. |
| Target | HLA-A*02:03&B2M&AFP (FMNKFIYEI) |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Alpha-fetoprotein (AFP), a specific liver cancer marker, T cells expressing AFP-CAR selectively degranulated, released cytokines, and lysed liver cancer cells that were HLA-A*02:01 /AFP while sparing cells from multiple tissue types that were negative for either expressed proteins.CAR T-cell immunotherapy targeting intracellular/secreted solid tumor antigens can elicit a potent antitumor response. |
| Molecular Mass | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result. |
| Sl Sequence | Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | AAA03604.1(HLA-A*02:03)&P61769(B2M)&FMNKFIYEI |
| Amino Acid Sequence | Recombinant Human HLA-A*02:03&B2M&AFP (FMNKFIYEI) Tetramer Protein is expressed from HEK293 His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:03), Ile21-Met119(B2M) and FMNKFIYEI peptide. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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