Biotinylated Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein (MHC-HM462B)
SKU: BTL-KB-P-02815 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | MHC-HM462B |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Biotinylated Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M) and AVVGASGVGK peptide. |
| Target | HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | RAS (H-ras, K-ras, and N-ras), as the second largest mutated gene driver in various human cancers, has long been a vital research target for cancer. Its function is to transform the extracellular environment into a cascade of intracellular signal transduction. RAS mutant protein regulates tumor cell proliferation, apoptosis, metabolism and angiogenesis through downstream MAPK, PI3K and other signaling pathways. |
| Molecular Mass | The protein has a predicted MW of 50.25 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and AVVGASGVGK peptide |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | AAV53343.1(HLA-A*11:01)&P61769(B2M)&AVVGASGVGK |
| Amino Acid Sequence | Recombinant Biotinylated Human HLA-A*11:01&B2M&RASL11B (AVVGASGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M) and AVVGASGVGK peptide. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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