Biotinylated Human MMP-9 Protein (pro form) (MMP-HM409B)
SKU: BTL-KB-P-02914 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | MMP-HM409B |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Biotinylated Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. The protein needs to be activated by APMA for an activated form.It contains Ala20-Asp707. |
| Target | MMP-9 |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead to impaired healing. Inappropriate expression of MMP9 also contributes to impaired re-epithelialization. Previously we demonstrated that FOXO1 was activated in wound healing but to higher levels in diabetic wounds. To address mechanisms of impaired re-epithelialization we examined MMP9 expression in vivo in full thickness dermal scalp wounds created in experimental K14. |
| Molecular Mass | The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 95-105 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Ala20-Asp707 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | P14780 |
| Amino Acid Sequence | Recombinant Biotinylated Human MMP-9 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. The protein needs to be activated by APMA for an activated form.It contains Ala20-Asp707. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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