Human PBK/TOPK Protein (PBK-HB101)
SKU: BTL-KB-P-03164 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | PBK-HB101 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human PBK/TOPK Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the C-terminus.It contains Met1-Val322. |
| Target | PBK/TOPK |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE |
| Scientific Background | T-lymphokine-activated killer cell-originated protein kinase (TOPK, also known as PDZ-binding kinase or PBK) plays a crucial role in cell cycle regulation and mitotic progression. Abnormal overexpression or activation of TOPK has been observed in many cancers, including colorectal cancer, triple-negative breast cancer, and melanoma, and it is associated with increased development, dissemination, and poor clinical outcomes and prognosis in cancer. Moreover, TOPK phosphorylates p38, JNK, ERK, and AKT, which are involved in many cellular functions, and participates in the activation of multiple signaling pathways related to MAPK, PI3K/PTEN/AKT, and NOTCH1. |
| Molecular Mass | The protein has a predicted MW of 37.61 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Met1-Val322 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | Baculovirus-Insect Cells(Sf9) |
| Gene Id | Q96KB5-1 |
| Amino Acid Sequence | Recombinant Human PBK/TOPK Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the C-terminus.It contains Met1-Val322. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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