Product Description
| Cat Number | PGA-RY101 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Rat PDGF-AA Protein is expressed from Yeast with His tag at the N-terminus.It contains Arg86-Lys204. |
| Target | PDGF-AA |
| Host | Rat |
| Purity | > 90% as determined by Bis-Tris PAGE |
| Scientific Background | PDGF (Platelet-derived growth factor) is one among numerous growth factors that regulate cell growth and division. The PDGF family forms five dimeric ligands, PDGF-AA, PDGF-BB, PDGF-CC, PDGF-DD, and PDGF-AB, which activate cognate receptor tyrosine kinases PDGFR-α and PDGFR-β. Upon binding to its tyrosine kinase receptor PDGF-αα, PDGF-AA stimulates the proliferation of osteoblastic cells and may exert autocrine and paracrine effects in regulating bone-forming processes. PDGF-AA is the most well characterized mitogen that is not restricted to any kind of tumor cell type but was seen in osteoblastic cells as well as in pleomorphic tumor elements. |
| Molecular Mass | The protein has a predicted MW of 14.75 kDa. Due to glycosylation, the protein migrates to 17-22 kDa and 25-45 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Arg86-Lys204 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | Yeast |
| Gene Id | P28576-1 |
| Amino Acid Sequence | Recombinant Rat PDGF-AA Protein is expressed from Yeast with His tag at the N-terminus.It contains Arg86-Lys204. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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