Product Description
| Cat Number | PLD-HM104 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human PLD4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp52-Gly506. |
| Target | PLD4 |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. PLD4 could be involved in the activation process of M1 phenotype macrophages. |
| Molecular Mass | The protein has a predicted MW of 51.1 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Trp52-Gly506 |
| Solubility | Dissolve the lyophilized protein in 50mM MES, 100mM NaCl (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | Q96BZ4 |
| Amino Acid Sequence | Recombinant Human PLD4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp52-Gly506. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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