HRSV (B, strain 18537) Pre-fusion glycoprotein F0 Protein (RSV-VM1RB)
SKU: BTL-KB-P-03361 |
Host: HRSV (B, strain 18537) |
Brand: Kactus Bio
Product Description
| Cat Number | RSV-VM1RB |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant HRSV (B, strain 18537) Pre-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus. |
| Target | Pre-fusion glycoprotein F0 |
| Host | HRSV (B, strain 18537) |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body. |
| Molecular Mass | The protein has a predicted MW of 56.50 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Amino Acid Sequence | Recombinant HRSV (B, strain 18537) Pre-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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