Human SEMA3A/Semaphorin-3A Protein (SEM-HM13A)
SKU: BTL-KB-P-03391 |
Host: Human |
Brand: Kactus Bio
Product Description
| Cat Number | SEM-HM13A |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human SEMA3A/Semaphorin-3A Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn21-Val771. |
| Target | SEMA3A/Semaphorin-3A |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain. |
| Molecular Mass | The protein has a predicted MW of 87.7 kDa. Due to furin cleavage site, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Asn21-Val771 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | Q14563 |
| Amino Acid Sequence | Recombinant Human SEMA3A/Semaphorin-3A Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asn21-Val771. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
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