Product Description
| Cat Number | TIE-HM201 |
|---|---|
| Category | Recombinant Protein |
| Pack Size | 100UG |
| Description | Recombinant Human TIE2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Leu748. |
| Target | TIE2 |
| Host | Human |
| Purity | > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
| Scientific Background | Angiopoietin-1 (Ang-1) is the primary agonist for Tie2 tyrosine kinase receptor (Tie2), and the effect of Ang-1-Tie2 signalling is context-dependent. Deficiency in either Ang-1 or Tie2 protein leads to severe microvascular defects and subsequent embryonic lethality in murine model. Tie2 receptors are expressed in several cell types, including endothelial cells, smooth muscle cells, fibroblasts, epithelial cells, monocytes, neutrophils, eosinophils and glial cells. |
| Molecular Mass | The protein has a predicted MW of 82.09 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. |
| Sl Sequence | Ala23-Leu748 |
| Solubility | Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
| Appearance | Lyophilized |
| Endotoxin Levels | Less than 1 EU per μg by the LAL method. |
| Expression System | HEK293 |
| Gene Id | AAA61139.1 |
| Amino Acid Sequence | Recombinant Human TIE2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala23-Leu748. |
| Storage | -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| View Document | |
| Note | The product is for research use only |
Share Item: