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Recombinant Proteins Uses and Applications
Recombinant proteins are recombinant DNA encoded proteins which have been cloned in an expression vector system supporting the expression of the gene and mRNA translation. The modifications done by the recombinant DNA technology leads to expression of a mutant protein. These proteins are manipulated form of native protein. Recombinant proteins are synthesized by genetic engineering which is also known as gene splicing or recombinant DNA technology. BTL Biotechno Labs Pvt. Ltd. is an authorized distributor of Recombinant proteins in India and related products.
What are vectors?
Purification and expression of a protein can sometimes be quite complicated &time-consuming; therefore an additional tag is used in addition to the specific DNA sequence which will facilitate the purification & expression of the recombinant protein.
Recombinant Proteins Vs Natural Proteins - Advantages of Recombinant Proteins
Recombinant Proteins have various advantages over natural proteins in terms of research:
- They can be synthesized by recombinant technology
- The protein yield is very high
- Batch to batch variations can be controlled.
- Protein do not contain any animal contaminants
- Amin acid sequences can easily be altered
- Unnatural amino acid can easily be incorporated
Types of Expression system
The strategy for expressing recombinant protein involves the transfection of DNA vector containing recombinant DNA with in a particular cell type which are then further cultured that leads to translation of the protein of interest. Hence, choosing an appropriate protein expression system is the key to the success of recombinant protein expression. Various factors are taken into consideration while selecting the expression system like: target protein property, intended application, yield and cost. Moreover, there are various challenges that also exist in protein expression projects, especially in case of High Molecular weight protein, membrane protein, nuclear protein and proteins with heavy post-translational modifications. We are also a leading ELISA Kit Distributor In India.
Process of production of Recombinant Proteins
There are various types of expression system commonly used these days:
- Bacterial Expression system
- Mammalian Expression System
- Yeast Expression system
- Insect Expression system
- Cell free system
Comparison of different expression system
| Expression System | Advantages | Challenges | Applications |
|---|---|---|---|
| Bacterial | Cost effective Rapid Expression Easy scale up | Difficult to express high MW proteins Lack of eukaryotic post-translational modifications | Antibody generation Cytokines Enzymes Structural analysis |
| Yeast | Cost effective Rapid Expression High Yield Eukaryotic protein processing | Growth conditions may require optimization Improper Glycosylation | Lower MW proteins Cytokines Enzymes |
| Insect | High-Capacity generation Soluble proteins Suitable for toxic proteins Post-translational modifications similar to Mammalian | Lack of Partial Glycosylation More demanding culture conditions Production of baculovirus vectors is time-consuming | Cytoplasmic proteins Toxic proteins Expression of Protein complexes Kinases |
| Mammalian | Highest level protein processing Soluble protein Lower endotoxin Better bioactivity Stable expression | More demanding culture conditions High cost | Secretory proteins Antibody fragments Recombinant antibodies Protein interactions |
| Cell free | Open system, able to add unnatural components Fast expression Simple format | Degradation of protein by exogenous proteases | Antigen Production Translational inhibitor screening Functional assays |
Applications of Recombinant proteins
Recombinant proteins have various application in the field of therapeutics and other research. They prove to be an important tool in understanding protein-protein interactions and further are used to develop enzymatic assays. They have proven importance in various research techniques like ELISA, western blot and immunohistochemistry (IHC). Recombinant proteins of various biomarkers have a critical impact on research these days which includes interleukins, hormones, blood clotting factors, interferons, growth factors, tumour necrosis factors, thrombolytic drugs, and further in the field of diseases like cancer, diabetes, congestive heart failure, dwarfism, myocardial infarction, thrombocytopenia,cerebral apoplexy, rheumatoid arthritis, multiple sclerosis, neutropenia, anaemia, hepatitis, asthma, Crohn’s disease, SARS-CoV-2 and many more.
Our Range of recombinant Proteins
BTL Biotechno Labs Pvt. Ltd. is an authorised supplier of Recombinant Proteins in India. You can buy Recombinant Proteins online through our website.
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Why BTL Biotechno Labs Pvt Ltd – Best recombinant proteins Distributor In India?
BTL Biotechno Labs Pvt. Ltd. provides more than 1,40,000 recombinant proteins covering various targets, multiple tags with high purity and lower endotoxin levels in multiple expression systems with the best price and fastest delivery at your doorstep. Along with this we also offer Custom Recombinant Protein service to express purified proteins.
Looking for the Best Recombinant proteins distributor in India to buy Recombinant proteins online? BTL Biotechno Labs Pvt. Ltd. serving a huge range of Recombinant proteins products across India
How To Buy Recombinant Proteins Online @ BTL Biotechno Labs Pvt. Ltd. ?
We are a leading supplier of recombinant proteins in India. You can place an order and buy Recombinant proteins online from our official website: https://biotechnolabs.com
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