The clinical value of circulating, cell-free DNA (cfDNA) for non-invasive disease detection, diagnosis, and monitoring is significant. The size distribution peak of cfDNA, which are typically short DNA fragments with the potential to be used as a screening tool for cancer and tumours as well as foetal DNA research, lies at 170 base pairs (bp). Since circulating DNA is typically present in small amounts, it can be difficult to extract it. Accurate, reliable techniques are required to recover the less common cfDNA with greater sensitivity. Omega Bio-tek has developed cfDNA extraction kits for automated, high-throughput workflows utilising magnetic beads (Mag-Bind® cfDNA Kit, M3298) as well as manual processing using silica spin columns (E.Z.N.A.® # Circulating DNA Kit, D3091). It further helps in isolating the concentrated cfDNA suitable for a varied range of downstream applications, including qPCR and next-generation sequencing, with adjustable sample inputs (1-4 mL for column-based; 0.5–4 mL for magnetic bead-based). The uniquely formulated binding buffer allows for large sample volumes to be processed in automated formats which allow for 4 mL of serum or plasma to be processed in a single well in 24-well plate format. The magnetic response of the Mag-Bind® Particles CH allows for fast magnetization during steps requiring high volumes, and the high binding capacity allows for reduced amounts of magnetic particles required, thus reducing the elution volume down to 50 µL for 4mL serum or plasma samples.

Kit components: Mag-Bind® Particles CH, DS Buffer, JSB Buffer, GT7 Buffer v1.1, SPW

We, BTL Biotechno Labs Pvt. Ltd., as the authorized Indian channel partner of Omega Bio-tek distribute their Mag-Bind® cfDNA Kit, M3298 & E.Z.N.A.® Circulating DNA Kit, D3091 for the extraction and quality analysis of DNA from varied samples. Since 1999, Omega Bio-tek has earned a reputation for delivering innovations in nucleic acid purification. Omega Bio-tek manufactures high-quality DNA and RNA isolation products for clinical and basic research, biotechnology, genomic and agricultural applications and offer superior nucleic acid extraction technologies, utilizing an array of techniques including magnetic beads, silica membranes, and salting-out.

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