What are antibodies? Let understand this first
An antibody, also called Immunoglobulin is a Y shaped molecule that consists of 4 chains: 2 heavy and 2 light. They are proteins produced by the body’s immune system in response to encounter with antigen, providing protection against the future encounter with the antigen. We BTL Biotechno Labs Pvt Ltd the best manufacturers of Antibodies in the industry are here to advance the research areas utilising antibodies as their main tool including Cancer, Epigenetics, Inflammation, Immunology, Cell Signalling, Cell structure, Neuroscience, Diabetes, Apoptosis, Infectious Disease, Stem cell research etc. There are five different types of antibodies: IgG, IgM, IgD, IgA and IgE.
Primary antibodies are those antibodies that interact with the antigen and binds to it.
Different types of Antibodies available at BTL Biotechno Labs Pvt Ltd
- Polyclonal antibody
- Monoclonal antibody
Polyclonal antibodies are the heterogeneous population of antibodies which target many epitopes of the same antigen where as Monoclonal antibodies are the homogenous population of the antibodies targeting single epitope of the antigen.
Polyclonal vs Monoclonal
|Polyclonal Antibodies||Monoclonal Antibodies|
|Mixed population of antibodies||Single Population of antibodies|
|May bind to different areas of the target||Binds to only 1 region of the target.|
|High Cross reactivity||Low cross reactivity|
|Medium specificity||High Specificity|
|Rapid Production||Long Production time|
Procedure for generation of Polyclonal antibodies:
- Injection of antigen to the animal
- Activation of B cells by the antigen
- Plasma B cells produces Antibodies
- Collection of antibodies containing serum
- Purification of antigen specific antibodies
BTL Biotechno Labs Pvt Ltd – Trusted Antibodies Supplier in India
BTL Biotechno Labs Pvt Ltd is an authorised Supplier of Antibodies in India. Our team is dedicated to deliver you antibodies related products as per your custom requirement and provide good after sales support.
Procedure for the generation of Monoclonal antibodies in India:
- Injection of antigen into the animal and preparation of myeloma cells
- Isolation of spleenocytes from the animal and collection of myeloma cells
- Fusion of spleenocytes and Myeloma cells to form hybrid cells
- Separation of hybrid cells followed by their proliferation
- Screening for monoclonal antibody
- Scale up production
There are two categories of Monoclonal Antibodies: Rabbit monoclonal antibodies and Mouse monoclonal antibodies. Rabbit monoclonal antibodies have proved to be better than mouse monoclonal antibodies because of the following reasons:
- High specificity
- Higher affinity up to picomolar range.
- Low background Noise
- Ideal for IHC
- Good for Post translational modification detection
Secondary antibodies are needed for the detection of primary antibodies. They are named as ‘Anti-antibody’. They offer increased sensitivity due to multiple binding to a single primary antibody. In addition, a given secondary antibody can be used with any primary antibody with the same isotype and target species. Secondary antibody combines enzyme or fluorescent, and indirectly detects the target protein by binding with the primary antibody, thus amplifying the signal and improving the sensitivity of detection.These labelled antibodies can be used to isolate and purify a protein of interest from a complex mixture, usually cells, tissues or whole organisms.
The most common enzymes used for antibody conjugation is Horseradish peroxidase (HRP). Apart from this conjugation by fluorescent molecules is also available including molecules like: FITC, PE, APC and PerCP.
Applications of Antibodies
Antibodies are essential for various biomedical research applications:
Selecting an Antibody
The researcher or scientist should keep following things in mind while selecting an antibody:
- Identification of the target
- Species Reactivity
- Antibody Host Species, Isotype, and Subclass Considerations
- Antibody Clonality Considerations
- Antibody Production Considerations
- Antibody Sensitivity & Specificity
Loading control antibodies
Loading controls are antibodies that are expressed by the ‘housekeeper genes’. They exhibit high level, constitutive expression in a specific cell type or sample. The band density these loading controls can be used for thequantificationof protein expression when lanes have been unevenly loaded. These controls aid in the assessment of equal transfer between gel and membrane in western blot experiments. These are crucial in producing reliable results when comparing levels of protein expression in different samples. These loading controls need to have different molecular weight to protein of interest (to allow clear and separate bands). They are available for various applications including IHC, IF, Western Blotting, ELISA etc.
- For Whole cell, Cytoskeleton : GAPDH, α tubulin, β tubulin, β actin
- For Nuclear: Histone H3, Lamin B1
- For Mitochondria: VDAC1, COXIV, TOMM20
Your search for the best antibodies in India ends at BTL Biotechno Labs Pvt Ltd as BTL Biotechno Labs Pvt Ltd is a channel partner of multiple renowned global antibody manufacturers and suppliers. BTL Biotechno Labs Pvt Ltd has been supplying a wide range of Primary, Secondary, Monoclonal, and Polyclonal antibodies to the Indian Scientific community for many years.
How to order Antibodies from BTL Biotechno Labs Pvt Ltd ?
To order Antibodies from you have follow these steps:
- Visit to our official website: https://biotechnolabs.com
- Send us the enquiry of Antibodies